Microlenses Fabricated by Two?Photon Laser Polymerization for Cell Imaging with Non?Linear Excitation Microscopy

Non-linear excitation microscopy offers several advantages for in-vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due scattering and spherical aberrations induced on focused beams by the tissue. The use of low numerical aperture objectives pass through outer layers skin, together with high dioptric power microlenses implanted close observation volume, beneficial reduction optical aberrations. Here, Fibroblast cell culture plano-convex used non-linear biological are developed tested. as single lenses or multiplexed in array. A thorough test wavefront is reported modulation transfer function profile. Magnified fluorescence images retrieved coupled commercial two-photon scanning microscopes. signal-to-noise ratio not substantially affected magnification adjusted changing relative position microlens array microscope objective immersion medium. These results opening way application micro-optics inspection processes.